Highly inclined and laminated optical sheet hilo microscopy is an optical technique that employs a highly inclined laser beam to illuminate the sample with a thin sheet of light that can be. Highly inclined and laminated optical sheet hilo microscopy significantly improves the signal to background ratio by increasing the image intensity and decreasing the background intensity. Highly inclined and laminated optical sheet hilo microscopy is an optical technique that employs a highly inclined laser beam to illuminate the sample with a thin sheet of light that can be scanned through the sample volume 1 hilo is an efficient illumination technique when applied to fluorescence imaging of thick samples owing to the confined illumination volume that allows high contrast.
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