High Content Screening Definition

Extreme conservatism is used in high throughput screening due to the large number of tests performed.

High content screening definition. In high content screening phenotypes are defined as a complex description of the cellular or organismal response toward an experimental perturbation. High content screening hcs also known as high content analysis hca or cellomics is a method that is used in biological research and drug discovery to identify substances such as small molecules peptides or rnai that alter the phenotype of a cell in a desired manner. Limitations edit the constant factor 3 in the definition of the z factor is motivated by the normal distribution for which more than 99 of values occur within 3 standard deviations of the mean. Automated high content screening platforms are capable of producing thousands of images per day.

Our high content screening portfolio consists of thermo scientific cellinsight cx5 cx7 led and cx7 lzr hcs platforms and hcs studio software designed to provide you cutting edge images and data in no time. At present hcs is primarily a fluorescence imaging method. High content screening hcs also known as high content analysis hca or visual screening is a method that is used in biological research and drug discovery to identify substances such as small molecules peptides or rnai that alter the phenotype of a cell in a desired manner. The opera phenix plus high content screening system is the premier confocal solution for today s most demanding high content applications.

In certain cases phenotypes have clear cell biological correlates such as large cells mitotic cells and apoptotic bodies but phenotypes can also be subtle changes in the distribution of. 1 2 hence high content screening is a type of phenotypic screen conducted in cells. High content screening hcs also known as high content analysis or hca is an approach to cell biology that combines automated imaging and quantitative data analysis in a high throughput format suitable for large scale applications such as drug discovery and systems biology. The challenge is to use appropriate analysis methods to extract the maximum amount of biologically relevant information from these images.

In this article we assess whether hcs is as high content as it can be. Explore our high content screening platforms that are designed to provide exceptional resolution for subcellular detection automated. Used extensions nodes extensions nodes. Target based high throughput screening hts has recently been critiqued for its relatively poor yield compared to phenotypic screening approaches.

In this article we summarise the basic concepts of image analysis and highlight examples of both open source and commercial software that are available for use. Of course parameters and methods have to be adjusted for your problem but this is a good starting point. Drawing on over a decade of experience with the industry leading opera and opera phenix system the opera phenix plus is designed for high throughput high content assays phenotypic screening assays using complex disease models such as live cells.